This work addresses the possibility of detecting the risk factor of atherosclerotic plaques using diagnostic ultrasound. The aim is to show methods to detection the risk factor of plaque what are recently known. The first part describes role of the diagnostic ultrasound and its modes and the artifacts that can occur. The second part describes atherosclerosis and its risks followed by displaying of the plaques in B-images. The last part describes the investigated data and a pilot study with developed MATLAB-based software with cooperation of an experienced sonographer to compare features in B-MODE and from histological patterns. Also will be discussed and improved the adaptability of the software for new tissues.