In each country, the conditions for doing business and the business environment itself are different, favourable or unfavourable for different business sectors. Not only for the development of the region is an important carrier of active business activity. An important aspect is also the possibility of choosing a place for business activities of the given business entities, who are looking especially for locations that are located near the backbone road or rail transport. The aim of this article is to identify and describe the factors that influence the possibility of doing business in the statutory city of Karviná. In connection with the aim of the work is carried out a questionnaire survey focused on small and medium enterprises in the city of Karviná.