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The width (W), root mean squared amplitude (Rs) of lower and upper kHz quasi-periodic oscillations (QPOs) from accreting neutron stars vary with frequency. Similarly, the QPO frequency varies with the source count rate (S). Hence, the significance of a QPO, scaling as S x Rs^2/W^(1/2) will also depend on frequency. In addition, the significance also scales up with the square root of the integration time of the Fourier power density spectrum (T). Consequently, depending on the way data are considered, kHz QPOs may be detected only over a limited range of their frequency spans or detected predominantly at some frequencies, leading potentially to biases in the observed distributions of frequencies or frequency ratios.