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Background: The basic prerequisite for initiating dialysis is to ensure a quality vascular access, which will allow a patient to be regularly connected to the extracorporeal circuit. The arteriovenous joint (AVF) has remained the gold standard and the most common choice for many years. Aim: To find out how dialysis patients assess pain when a needle is inserted into the arteriovenous fistula, and whether they know and use pain relievers. Methods: The research group consisted of 61 dialysis patients. The average age was 61.7 years. A non-standardized questionnaire was used for a data collection and numerical scale to monitor pain. Results: We used NRS to monitor the pain when inserting the needle into the AVF. Mild pain was assessed by 32 (52.5%) respondents, moderate pain was assessed by 14 (22.9%). Injection site pain during the dialysis was not felt by 37 (60.6%), mild pain was reported by 22 (36.1%) and moderate pain was reported by 2 (3.3%). After the completion of the dialysis and treatment of the injection site, pain intensity was assessed using NRS, where 41 (78.6%) respondents did not feel pain at the injection site, mild pain was reported by 4 (6.6%) and 9 (14.8%) reported moderate pain intensity. Patients do not use local anesthetics. 34 (55.7%) do not know that local anesthetics exist, 13 (21.3%) have some awareness and 7 (11.5%) are aware of them. Using Spearman's correlation coefficient, we analyzed pain by NRS during cannula lining into the arteriovenous fistula during injection and after dialysis at the injection site. There was a significant negative dependence of NRS during the insertion of the cannula on the length of inclusion in the dialysis program, r = -0.29. Conclusion: The use of NRS showed the presence of mild pain in half of the patients. An association between dialysis duration and AVF puncture pain was found.