The chapter deals with factors that may affect the willingness to do business. Factors such as business economics knowledge, desire for freedom and success, appetite for risk-taking and organizational skills are examined as the pillars of potential businesses. Analysis of the factors shows the influence on the business establishment. The chapter is based on primary data collection from a recent survey from the Czech Republic and on comparison with international resources focused on aspects which influence the business establishment. The results indicated relationships between above-mentioned factors and their effects on the business establishment. Implications of these results, as well as directions for future research, are discussed.