The use of an agricultural land in the Czech Republic is one of the increasingly topical topics. In recent years, an agricultural land in the country has been steadily declining and becoming a valuable natural resource. In recent years, agricultural land has given way mainly to construction activities, most often to the construction of development projects on the outskirts of larger towns and the construction of new economic entities. In this case, we are talking about inefficient land use, because in the case of suburbanization, the land is stopped on greenfields. A possible solution is to find the effective use of abandoned buildings and areas in the given places, the so-called brownfields, which are supported in recent years by individual ministries in the Czech Republic, which deal with the situation within the usability of individual grant titles. The presented article aims is to determine the impact of soil sealing on an agricultural land in individual NUTS 3 regions in the Czech Republic. For the purposes of the article, data on the state of the soil were obtained through the Czech Surveying and Cadastre Office. Other secondary data for the needs of the article were obtained through the Czech Statistical Office.