In the territory of the Czech Republic, the existence of abandoned buildings and premises has been more than evident. This phenomenon was predominantly caused by the transformation of the economy after 1989. The situation of abandoned buildings and premises on the territory of the Czech Republic has been dealt with mainly by the CzechInvest agency, which manages the National Brownfield Database. The paper is intended to compare data about the existence of brownfield sites on the territory of MEC in selected regions of the Czech Republic. The data about abandoned buildings and premises was compiled through the National Brownfield Database, when the authors analysed 4 regions on the NUTS 3 level (South Moravian, Moravian-Silesian, Olomouc and Zlín regions) for 2021. The relevant findings were then subjected to deeper examination on the level of MEC in individual regions. The examined attributes included the number, site, previous use and contamination. On the basis of the examined attributes in the relevant regions on the MEC level, it was found out that the previous activities of abandoned buildings and premises mainly included industrial and agricultural activities, and then former community facilities. On the basis of the results, the authors found that the ownership of abandoned buildings and sites was dominated by private ownership followed by public and combined ownership. Contamination of brownfields was detected in a small number of analysed brownfields. The findings based on the relevant paper can contribute to a deeper understanding of the situation concerning abandoned buildings and premises on the territory of the relevant regions in the relevant country.