In this paper, we explore the dynamics of test particles in spacetime around regular Bardeen black holes (BHs) in the novel four-dimensional Einstein-Gauss-Bonnet (4D EGB) theory. First, we analyze properties of the spacetime around the Bardeen BH with respect to the Gauss-Bonnet (GB) coupling parameter a, and we show the range of values of the BH charge and the parameter a that horizon exists as alpha/M-2 is an element of (-0.15869, 1) and g/M is an element of (0,0.886247), respectively. We also investigate the effects of the magnetic charge of the regular BH on innermost stable circular orbits (ISCOs) radius and efficiency of energy release from infalling test particles to the regular Bardeen BH in 4D EGB gravity. Moreover, fundamental frequencies such as Keplerian and harmonic oscillations are also studied with the applications of quasi-periodic oscillations (QP0s) where we proposed a method to test gravity theories through data from astrophysical observations of twin-peak QPOs in relativistic precession (RP) model. Finally, we show the dependence of the distance from the central BH to the orbit where twin-peak QPOs shine from the spin of Kerr BH, magnetic charge of Bardeen and Reissner-Nordstrom (RN) BHs, and GB coupling parameter.