This paper aims to evaluate the influence of investment and R&D expenditures on the performance of large companies that are headquartered in the Europe using regression analysis of the generalized method of moments. The independent control variables are GDP per capita and the interest rate represented by the yield of 10-year state bonds. The sample period is 2010–2018. Our result shows that the growth of R&D expenditures and investments negatively affected the profitability ratios of the firms in the year of implementation. While concentrating on the macroeconomic control variables, the results support an assumption about the positive effects of GDP on the majority of the net income, ROA and ROE estimates. On the other hand, negative effects of interest rates were found.