As Czech export is widely considered the key to the economic development of Czechia, this chapter explores the relationship between microeconomic profitability among companies in selected TOP10 export industries and the macroeconomic development of the export itself. An investigation was carried out to compare the differences caused by the COVID-19 pandemic. In addition, the comparison is developed according to the size and concentration of ownership among exporting companies. Annual data are obtained from the Bureau van Dijk Orbis database to analyse profitability among 4,283 companies in 10 NACE industries from 2012 to 2021. We have obtained encouraging results, demonstrating that not only those less profitable companies affected export development. However, in general, our results emphasise the importance of those less profitable medium-sized companies for Czech export, within the manufacture of machinery and equipment, and the manufacture of motor vehicles in particular.