The presented contribution deals with the analysis of the relation between the regional university school system and the business sector in the field of information and communication technologies in two selected regions of the Czech Republic. The South Moravian Region and the Moravian-Silesian Region are the only two regions, with the exception of the capital city of Prague, in which the field of information and communication technologies has been positively developing and concentrating for a long period of time. The reason for this is the fact that the business sector focused on producing articles or providing service in the sphere of information and communication technologies is closely connected to the local universities, specifically those faculties of technology that generate the required trained staff and improve their qualification potential. The contribution is based on the secondary data of the Ministry of Education, Youth and Sports in the Czech Republic and at the same time proceeds from the primary research on the issue of the biased relation between the sectorally oriented ICT companies toward the educational institutions and their requirements on the employees.