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Currently, a rail transport in the EU undergoes by significant reforms, which aims to liberalize the market for rail transport services, to eliminate the monopoly of the national transport and open up free access of independent operators to transport infrastructure and systematic improvement of the public transport services. The article discusses the influence of important globalization factor - the shaping of competition and creation of a competitive environment in offer of rail-passenger transport in the Czech Republic. On the rail market entered a private carriers, created are a new business conditions for the providing of rail passenger transport. This article aims to map and define the competitive environment in rail passenger transport in the Czech Republic, to assess the impact of the created competitive environment on the shaped offer, a quality and producing of transport services by the largest providers of passenger rail transport in the Czech Republic, from private and public sector. For evaluation the competitive positions of the suppliers on the public services are used a method of SWOT analysis, cost analysis and pricing analysis, comparative analysis and trend analysis. Analyzed are a primary data from public surveys and secondary data from available sources. In the end is evaluated impact on the passenger satisfaction through the survey of the offer and quality of the services, which are provided to the passengers and as well as the opportunities for exploitation the rail passenger transport in tourism.