Brownfield regeneration is one of the key measures for sustainable development and it combines three pillars – economic, environmental and social. The aim of this paper is to identify interlinkages between strategic, financial and regional frameworks of brownfield regeneration in the Czech Republic. The first part of the paper consists of literature review associated with brownfield regeneration. The second part of paper focuses on practical aspects of brownfield regeneration in the Czech Republic during the period 2000 to 2020. This empirical part is based on analysis of strategic documents, financial instruments and regional disparities. In this paper, national level and regional level data were applied (7 NUTS II regions excluding the region of the capital city Prague). Data summarizing the drawn funds or allocated financial amount to support the regeneration of brownfields from EU funds were obtained from secondary sources mainly (national strategies, websites of individual operational programs or national programs). The results show certain time delay in solving this issue in this post-socialistic country in comparison with Western European countries. Data also indicate noticeable differences between regions, both in terms of the number of brownfields and their regeneration, especially in terms of a number of projects or financial resources. Another finding is that the Czech Republic significantly improved the strategic planning of brownfield regeneration and as this problem is cross-sectional more institutions are involved both in strategic and financial dimension.