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Objective: The objective of the present study was to determine the level of cooperation between parents and nurses in an inpatient paediatric care setting. Methods: A quantitative design was applied in the present research with a standardized questionnaire (The Nurse Parent Support Tool, Miles, 1999) to determine the level of bio-psychosocial support offered to children and their parents by the nurse professional during hospitalization. The research sample comprised a total of 108 respondents (81 parents and 27 nurses). The survey was conducted in a paediatric and neonatal unit of a selected hospital in the Moravian-Silesian Region. Statistical tests were evaluated at a significance level of 5 %. Results: Data analysis suggests that co-operation between parents and nurses is on a high level. The best co-operation of nurses and parents was represented by score 5. Mean score reported by parents was 4.42±0,69, by nurses 4.40±0,55, respectively. Parents perceive a higher quality of care provided by nurses compared to nurses themselves (p=0.045). The lowest scores were identified in the domain of information level and communication with health care staff. Primarily, parents pointed KEYWORDS: hospitalization, child, parents, nurse, cooperation out to shortcomings in the way that nurses introduce themselves to both the children and their parents, and in the area of communicating nurses´ role toward the child. Conclusion: Nursing care of paediatric populations does not rely solely on the implementation of practical nursing procedures. It is also based on provision of complex and holistic nursing care to the child and its parents. Empathy and individualization in care are the only way of ensuring good cooperation of parents while their child is subject to nursing care in hospital settings.